Enterprise HealthDOTDepartment of Transportation
The federal department whose agencies regulate the medical fitness and drug/alcohol testing of safety-sensitive transportation workers.
Key facts
- Federal department overseeing transportation-safety agencies (FMCSA, FAA, FRA and more).
- Regulates safety-sensitive drug & alcohol testing under 49 CFR Part 40.
- Requires medical fitness exams for safety-sensitive transportation workers.
What it means
The U.S. DOT oversees modal agencies such as FMCSA (trucking), FAA (aviation), FRA (rail), FTA (transit) and PHMSA (pipelines). In occupational health, "DOT" most often refers to DOT-regulated drug and alcohol testing under 49 CFR Part 40 and to the physical examinations required for commercial drivers. DOT testing follows strict chain-of-custody procedures and is reviewed by a Medical Review Officer.
Frequently asked
What does 'DOT testing' mean?
It refers to drug and alcohol testing governed by DOT's 49 CFR Part 40 — with defined panels, chain of custody, certified labs and Medical Review Officer verification. It applies to safety-sensitive employees across DOT-regulated industries.
Which DOT agency handles truck drivers?
FMCSA, the Federal Motor Carrier Safety Administration, regulates commercial motor carriers — including DOT physicals and driver drug/alcohol testing. Other modes have their own agencies, such as the FAA for aviation and FRA for rail.
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