Testing & Screening

Drug & Alcohol Testing

Screening employees for prohibited substances, whether under DOT rules or an employer's own non-DOT policy.

Reviewed June 2026 by Enterprise Health

Key facts

  • Covers pre-employment, random, reasonable-suspicion, post-accident, return-to-duty.
  • DOT testing follows 49 CFR Part 40; non-DOT follows employer policy.
  • DOT testing requires chain of custody and MRO verification.

What it means

Workplace drug and alcohol testing spans pre-employment, random, reasonable-suspicion, post-accident and return-to-duty scenarios. DOT-regulated testing follows the strict 49 CFR Part 40 process — defined panels, chain of custody, certified labs and Medical Review Officer verification — while non-DOT testing follows employer policy. Managing collection sites, panels, results and the random pool is a core occupational-health workflow.

Frequently asked

What's the difference between DOT and non-DOT testing?

DOT-regulated testing follows the strict 49 CFR Part 40 process — defined panels, chain of custody, certified labs and Medical Review Officer verification. Non-DOT testing follows the employer's own policy.

What scenarios trigger workplace drug testing?

Commonly pre-employment, random, reasonable-suspicion, post-accident and return-to-duty testing. Managing collection sites, panels, results and the random pool is a core occupational-health workflow.

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